Biological Wetlab Technician in Gene Editing and Gene Translation Metabolism

The University of Luxembourg is an international research university with a distinctly multilingual and interdisciplinary character.

The Luxembourg Centre for Systems Biomedicine (LCSB) is an interdisciplinary research centre of the University of Luxembourg.
We conduct fundamental and translational research in the field of Systems Biology and Biomedicine - in the lab, in the clinic and in silico. We focus on neurodegenerative processes and are especially interested in Alzheimer’s and Parkinson’s disease and their contributing factors. The LCSB recruits talented scientists from various disciplines: computer scientists, mathematicians, biologists, chemists, engineers, physicists and clinicians from more than 50 countries currently work at the LCSB. We excel because we are truly interdisciplinary, and together we contribute to science and society.

**Your role**:
This research project has two main goals. The first is to use CRISPR to modify single point mutations in different genetic backgrounds in yeast, and to then grow these different types of yeast in different carbon environments and in different inhibitory chemicals, to see which mutations confer growth advantages. The second goal is to study the gene expression (i.e. the transcriptome and proteome) to understand the molecular pathways for _how_ those point mutations result in a change in growth. The first phase of the project will implement these DNA variants one-by-one, and afterwards the mutations will be implemented at higher orders to create yeast with more pronounced growth phenotypes, and to understand fundamental relationships in genetic regulation such as epistasis, additivity, and to experimentally test how reliable predictive models of DNA variant impact work at high throughput (e.g. tens of thousands of variants tested).

In brief, the applicant should be from a biology or bioengineering background. The project will be roughly 90% wetlab biology work, and 10% bioinformatician/data analyst work.

This project is funded in collaboration with a research group at EMBL/Stanford (Prof. Lars Steinmetz) and another at the Technical University of Munich (Prof. Julien Gagneur).

**Your profile**:

- a bachelor’s degree or equivalent in a biology; ideally with significant experience in yeast, but at least in cell lines
- a background in wetlab biology
- experience in extracting and performing basic quality checks on DNA, transcripts, and proteins
- in omics analysis of such data; particularly sequencing and mass spectrometry
- with population genetics, either in practice or in theory
- in programming and statistics; wetlab biology experience is preferred to this position compared to drylab skills
- with identifying nonlinear relationships in data, and/or with various modern methods for performing feature selection
- Fluency in oral and written English. Ability to communicate in French or German is a bonus, but the laboratory work at all three sites is entirely in English

**We offer**:

- Multilingual and international character. Modern institution with a personal atmosphere. Staff coming from 90 countries. Member of the “University of the Greater Region” (UniGR)
- A modern and dynamic university. High-quality equipment. Close ties to the business world and to the Luxembourg labour market. A unique urban site with excellent infrastructure
- A partner for society and industry. Cooperation with European institutions, innovative companies, the Financial Centre and with numerous non-academic partners such as ministries, local governments, associations, NGOs

**How to apply**:

- Applications should include:_
- Curriculum Vitae
- Cover letter
- General information:_
- Contract Type: Fixed Term Contract 18 Month
- Work Hours: Part Time 26.0 Hours per Week
- Location: Campus Belval
- Internal Title: Research support technician
- Job Reference: UOL07040

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